Sentence examples for measured threshold cycle from inspiring English sources

Quantification was finally achieved by plotting the measured threshold cycle (Ct) on the standard curve obtained with the serial dilutions of pDNA.

As amplification efficiency has been shown to change over the course of the reaction [ 29], we have based our analysis on the mid-point of the exponential phase to measure threshold cycles irrespective of platform, run or PCR reaction mix.

The amount of PCR products was measured by threshold cycle (Ct) values and relative mRNA levels are presented as unit values of 2̂[Ct GAPDH) -Ct(Zip4)].

Expression of genes was measured using threshold cycle values (CT).

The content of mtDNA was calculated using real-time quantitative PCR by measuring the threshold cycle ratio (ΔCt) of a mitochondrial-encoded gene (subunit II of cytochrome oxidase, forward 5'-CTTACAAGACGCCACATCAC-3', reverse 5'-GAATTCGTAGGGAGGGAAGG-3') versus a nuclear-encoded gene (peptidylprolyl isomerase A, forward 52-ACACGCCATAATGGCACTGG-32 52-ACACGCCATAATGGCACTGG-32 52-ACACGCCATAATGGCACTGG-32

Data analysis was performed on Mxpro ver 4.2 software to measure the threshold cycle (Ct) for each reaction.

The relative levels of amplified mRNA in each sample were quantified by measuring the threshold cycle (CT) values of target genes.

Reproducibility of the assay was evaluated by measuring the threshold cycle (Ct), i.e. the cycle with the first detectable increase in fluorescence, of a low run control on three consecutive days six times.

Relative quantification of the amplified gene levels in the bisulfite-converted genomic DNA sample was performed by measuring the threshold cycle (CT) values of ADHFE1 and β-actin (ACTB).

The relative expression level of the genes was calculated by measuring the threshold cycle (CT) (using version 1.1 software from the ABI Prism 7000 Sequence Detection System) and use of the comparative CT method as described by the provider (ABI Prism user bulletin #2).

MiRNA and mRNA levels were measured using Ct (threshold cycle).