In GFP-expressing lines, we also measured the expression levels of pch-2 using quantitative reverse transcription polymerase reaction (qRT-PCR).
To determine whether MEK1DD- and MEK2DD-expressing cells undergo an EMT, we examined the localization and measured the expression levels of various epithelial and mesenchymal markers.
We measured the expression levels of major proteins associated with the LFT, calculated BMI from measured weight and height and questionnaires were completed for alcohol consumption by the twins.
Dr. Johnson of Johnson & Johnson, working with Phase-1 Molecular Toxicology, measured the expression levels of 250 genes after exposing liver cells to 100 known toxic chemicals.
We measured the expression levels of selected genes involved in cell cycle.
To investigate the possible interaction between nitrogen and plant hormones, we measured the expression levels of the important genes responding to CK and SL pathways in the rice tiller bud with various transgenic lines.
To establish whether CD146 is required for the transcription of downstream β-catenin target genes upon Wnt1 treatment, we measured the expression levels of axin 2, cyclin D1 and c-Myc using RT-PCR (Fig. 1F).
To check whether Nrf2 was involved in NTG-induced TGVS activation, we measured the expression levels of nNOS and c-Fos in NTG-treated samples at 4 h after sulforaphane pretreatment.
To further validate the finding that PLP2-TM promotes autophagy, we measured the expression levels of endogenous LC3-I and LC3-II in cells following expression of PLP2-TM, in comparison with cells expressing the control vector, by Western blotting.
We also measured the expression levels of three costimulatory molecules (CD86, CD80 or CD40).
We measured the expression levels of cyclin D1 and cdk4 in Ishikawa cells treated with E2.
