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Mussels were individually measured (shell length) and separated into 10 mm size classes.
For comparison, clearance rates estimated from measured shell length (L) using the 'suction method' formula (Kiørboe and Møhlenberg, 1981; see also Riisgård, 2001a; Table 1 therein): CL l h−1) = 0.0012 L(mm)2.14 are also shown.
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After measuring shell lengths, the 15 inseminated and 30 control focals were isolated in perforated containers again.
Five linear shell measurements (shell length, shell width, spire height, and aperture length and width) commonly used in gastropod studies [ 25, 31, 34] were measured using Image-Pro Express.
On day 11 after measuring their shell lengths, they were sacrificed by injecting about 3mL of 50mM MgCl2 through their foot.
The individuals were measured for their shell length (L; maximum anterior posterior axis), shell height (H; maximum dorso-ventral axis), and shell width (W; maximum lateral axis) using a digital caliper to the nearest 0.1 mm.
In addition, the collected blood cockles were measured in terms of the shell length of each individual.
Thereafter, nine opihi were selected for each experimental group and their initial weight and shell length were measured.
After weighting, individual mussel shell length was measured, the soft tissue was scraped off from shells using a scalpel.
Seven mature Nautilus (four males and three females) were sacrificed for laboratory studies and their shells measured for total ventral circumference, shell length and number of chambers.
Shell length was measured at the initiation of the experiment and every 2 weeks subsequently to determine growth rate.
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