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The benefit of using CAI is that it can be computed for any gene with a codon frequency distribution, whereas experimentally measured protein expression or ribosomal loading data are often limited to relatively highly expressed proteins or transcripts.
In these experiments, we measured protein expression levels of all the NADPH oxidase subunits.
As an indicator of Nrf2 activity in vivo, we measured protein expression of NQO1 in lung homogenates.
In addition, we measured protein expression levels in six independent pools of tissue extracts, each containing two of the human or two of the chimpanzee samples, respectively, in two sets of experimental replicates, using ICAT profiling (Materials and Methods).
To determine if ventilator-induced reduction of Ang-1, Ang-2 and Tie2 mRNA also resulted in a reduction of Ang-1, Ang-2 and Tie2 protein, we measured protein expression of these mediators in total lung homogenates.
Thus, in order to show that skeletal muscle, and not phagocyte, NADPH oxidase is a major cause of oxidative stress in dystrophic muscle, we measured protein expression levels in young (18 19 day old) mdx mice before the onset of muscle damage and inflammatory cell infiltration and activation.
Similar(32)
Additionally, immunostaining was used to measure protein expression.
Such techniques are valuable to measure protein expression, solubility, activity, and native posttranslational modification events in heterologous systems.
It is majorly used to measure protein expression levels and the intensities of specific bands by using an online software tool (Gelquant.NET).
However, the ability to measure protein expression, produce proteins (usually in recombinant form) and to block receptor ligand interactions remains essential for elucidating the function of the molecules of interest.
The term protein expression is however very rarely used in this sense, but tends rather to be used in the context of translation or protein synthesis, which is semantically not correct and further leads to misunderstandings when attempts are made to measure protein expression by two-dimensional gel electrophoresis or liquid chromatography.
More suggestions(15)
demonstrated protein expression
assess protein expression
far protein expression
measured protein diffusivity
measured protein stability
measured protein content
measured protein degradation
measured protein denitrosylation
measured protein secretion
measured protein amount
measured protein synthesis
measured protein binding
measured protein carbonylation
measured isoform expression
measured gene expression
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