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Choroidal thickness was measured manually using manual calipers.
Morphometric body traits were measured manually using the VHX-1000 built-in measoftware software.
Automated measurement of CPT was not available, so CPT was measured manually using the software calipers on a foveal B-scan image that was selected from 3D image as the distance between ILM to the top of RPE.
Automated measurement of CPT was not available, so CPT was measured manually using the built-in calipers on a single B-scan and a foveal B-scan that was selected from 3D image as the distance between ILM to the top of RPE.
Root diameters were measured manually using a pair of callipers.
Length, height, and width were measured manually using Pico view software.
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For the first 38 days of the experiment (from 26/11/08 to 4/1/09), pH was measured manually twice daily using a pH electrode (WTW Multi 340i with sensorex glass probe).
Amylase-positive area/pancreas area was measured manually with ImageJ using the freehand selection tool.
From twenty sections across primary colorectal cancer, ten sections of normal colon mucosa, ten sections of normal liver and twelve sections of liver metastases a total of 100 samples of single CD3 positive T cells were measured manually for their area (using the Hamamatsu NDP viewer software).
Blood pressure was measured manually every 2 min using a sphygmomanometer.
After the start of the experiment, TEER values were measured manually every 2 3 days using an epithelial voltohmmeter (EVOM) coupled to an Endohm-6 measurement chamber (World Precision Instruments, USA).
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