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Samples were extracted and measured in quadruplicate.
Each shRNA transfection condition was performed and measured in quadruplicate cultures.
The absorbance of each well was read at 570 nm and normalized to wells containing no cells or added probe (measured in quadruplicate).
Cell survival/proliferation was measured in quadruplicate in 96-well plates by a spectrophotometric dye incorporation assay using sulforhodamine B (ICN Biomedicals, Irvine, CA, USA) after 96 h from treatment, as described before (Di Gennaro et al, 2009).
Antigen-specific T-cell responses, including IL-2 production and T-cell proliferation, were measured in quadruplicate samples of spleen cells (3 × 105 cells per well) cultured in the presence of 100 μg of collagen protein per milliliter.
Telomere length was measured in quadruplicate on 4 different plates with each replicate in the same well position on the polymerase chain reaction (PCR) plate by the monochrome multiplex quantitative PCR method, originally developed by Cawthon [ 21].
Similar(54)
Each experimental condition was measured in quadruplicates and each experiment was performed at least three times.
Fluorescence derived from daunorubicin or doxorubicin was measured in quadruplicates of 200 μl using a fluorescence plate reader (Gemini, Molecular Devices) at 480/575 nm.
The relative expression levels were calculated from three biological replicates, each measured in technical quadruplicate, after normalization to GAPC2 [ 51].
Samples were measured in ELISA in quadruplicate on at least two occasions on separate days.
Varying concentrations, [ N] T, of P-labeled pEndos (see Figure 7B,C) were incubated with [ P] T = 0.5 nM PP2A-B55, and the initial velocities, v, of P release were measured in (duplicate or quadruplicate) samples in two independent experiments.
Related(20)
evaluated in quadruplicate
assessed in quadruplicate
measured in underwear
measured in supernatant
measured in platelet
measured in capillary
measured in decibel
measured in reference
measured in sperm
measured in reaction
measured in batch
measured in practice
measured in tension
measured in space
measured in stool
measured in membrane
measured in triplicate
measured in quartz
measured in association
measured in absence
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