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Putative targets were validated in independent ChIP followed by q-PCR, while regulatory effects were measured in expression profiling experiments and confirmed by RT-qPCR.
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In this case, the list of top-scoring GO terms represents the important processes that were measured in the expression data.
Expression of genes was measured in triplicate and expression levels were calculated by the comparative Ct method (Applied Biosystems).
"Measuring the World" is elegant and measured in design and expression.
Using quantitative PCR, we measured fluctuations in expression of these clock genes over both diel and monthly spawning timeframes.
Since EZH2 is required for Polycomb silencing we measured differences in expression levels of Ezh2 in young and senescent MEFs by Q-RT-PCR (Fig. 1C).
Therefore, we measured changes in expression of Cyp1a1 and C yp1b1 as controls for AhR pathway activation.
(a) Reporter gene expression measured in luminescence/colony-forming units (CFU), relative to the minimum expression (mean±s.e.m., n=5).
qRT-PCR expression was measured in Ct values (PCR cycle at which expression was detected).
Targets were further categorized according to mean log2 expression (measured in RPKM).
The results were normalized to GAPDH expression measured in each sample.
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