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Glycemia were measured from tail vein blood using a Glucometer (Glucotrend Premium; Boehringer Mannheim GmbH).
At day 5 of the STZ injections, serum glucose was measured from tail vein blood using a glucometer.
Deactivation kinetics were measured from tail currents (−120 to −20 mV) following a prepulse depolarization to +60 mV.
Steady-state activation currents in the presence of 5 mM K+/135 mM Na+ or 140 mM K+ were measured from tail currents at +30 mV following prepulses ranging from −150 to −60 mV in 10 mV increments, which increased in duration by 850 ms with each more depolarized potential.
Blood samples were taken and circulating hVEGF-A165 and endogenous mVEGF-A164 levels were measured from tail vein plasma samples at various time points after the gene transfer with enzyme-linked immunoassays (R&D; Quantikine, human VEGF-A and mouse VEGF-A).
Serum insulin was measured from tail blood with an ultrasensitive mouse insulin ELISA kit (Mercodia, Sweden).
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Four days after injection, glucose concentration was measured from tail-blood samples via a Hemocue B glucose analyzer (Stockholm, Sweden).
Blood glucose was measured from tail-tip blood using an Ascensia Contour meter (Bayer, Berkshire, UK).
Two weeks later, the glucose level was measured from tail-vein blood, and mice with glycemia (over 200 mg/dL) were selected for the study [ 9].
At sacrifice, body weights were recorded and blood glucose concentrations and percent glycosylated hemoglobin were measured from tail-nick blood samples using a Lifescan glucose meter and Siemens DCA analyzer, respectively.
After 7 days of injection, the plasma glucose concentration was measured from the tail vein.
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