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DNA binding activity of the NF-κB subunit p65 was measured from cell lysates using a TransAM® Transcription Factor ELISA Kit (Active Motif, Carlsbad, CA, USA).
SIRT1 protein levels were measured from cell lysates using the Sirtuin 1 (human) (IntraCellular) ELISA Kit (Adipogen, San Diego, CA, USA).
The amounts of phosphorylated p38 MAPK, ERK1/2, JNK1, MEK1, and CREB were measured from cell lysates using specific human PathScan® ELISA Kits obtained from Cell Signaling Technologies (Beverly, MA, USA).
At all exposure times, NPC ROI spectra had greater mean peak wavelengths than those measured from cell ROIs (Additional file 1: Figure S6).
To quantify TF activity after 48 h, GLuc was measured in culture media and FLuc was measured from cell lysates.
Osteocalcin was measured from cell culture medium using Mouse Osteocalcin EIA Kit (Biomedical Technologies Inc., MA) according to the manufacturer's protocol.
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At the beginning (Stage 1 or S1), mRNA levels were measured from cells.
The corresponding spectra (only a subset is shown for clarity) measured from cells exposed to bare and PEGylated NPs differ in both peak wavelength and broadness.
Net BRET was this ratio minus the same ratio measured from cells expressing only the BRET donor (Rluc8).
DNA fragmentation was measured from cells according to the manufacturer's specification using DNA fragmentation assay kit (Cell Death Detection ELISA plus kit; Roche Molecular Biochemicals, Mannheim, Germany).
IL-1β production was measured from cells according to the manufacturer's specification using IL-1β assay kit (R & D system Inc, Minneapolis, MN, USA).
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measured from horizontal
measured from seafloor
measured from fossil
measured from space
measured from threshold
measured from outside
measured from load
measured from registration
measured from capillary
measured from start
measured from wind
measured from study
measured from room
measured from tail
measured from serum
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