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For cilia measurements, at least 50 randomly chosen cilia were measured (from base to tip) per cell line for each culture condition at 1000× magnification using a Nikon Coolsnap ES camera and Image-Pro Express 2000 software (Media Cybernetics, Inc .. RPE1, HK-2 or MEF cells were grown in conditions to stimulate cilia formation and then fixed for 10 min in 100% methanol on ice.
It is cupped by the deep Hawaiian Trough, which has led some authorities to call it the tallest (as opposed to highest) mountain in the world, as measured from base to peak.
Linear growth was measured from base to tip of the colony and survivorship was recorded as live or dead.
Plant height (PH) was measured from base of the plant to tip of the head in centimeter.
Length of metacarpal bone III of the non-dominant hand was palpated and measured from base to head by measuring tape to the nearest 5 mm.
PC length (visualized by acetylated tubulin staining) was measured from base to tip using ImageJ National Institutes of Healthh, Bethesda, MD); at least 100 cells were scored per condition.
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Root length was measured from root tip to hypocotyls base.
Root length was measured from the base of the stem to the longest root using a ruler and root fresh weight was measured using digital scales.
Horn measurements (mm): horn length was measured from the base of the horn, along the outer curvature to the tip; horn circumference was measured around the base of the horn at the point closest to the skull; horn growth was measured from the base of the skull, along the outer curvature to the first growth increment (Johnston et al., 2013).
Total height and number of internodes was measured from the base of the plant to the base of the inflorescence when all of the flowers were developed.
Culm width was measured from the base of the stem using a caliper.
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