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The measured fluorescences are shown in Table 2.
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For the plate reader data, we assume that each measured fluorescence is a linear sum of autofluorescence and the fluorescence from any fluorescent reporter present in the cells.
Fluorescence was measured on an arbitrary scale where the background fluorescence was zero, and the maximum measured fluorescence was equivalent to 1.
Measured fluorescence was normalized against GAPDH.
Measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
Devices that are used to measure fluorescence are fluorometers, and those that are employed to measure phosphorescence are phosphorimeters.
The relations of the measured fluorescence intensities were comparable between the two microscopes.
The measured fluorescence intensities were then plotted to determine a biodistribution profile.
However, instruments that measure GFP surface fluorescence are expensive.
Fluorescence was measured after each 72°C step.
Fluorescence was measured at 535/587 nm.
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