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We measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate to gain insight into the carbohydrate esterase activities of this hemicellulolytic rumen bacterium.
We measured expression and production of myoblast markers: paired box-7 (Pax7), myogenic differentiation factor-1 (MyoD), myogenin (MyoG), myogenic factor-6 (Myf6), and myosin heavy chain (MyHC).
To examine the role of α7β1 signaling, we measured expression and production of α7, α5, and β1 and myoblast markers in wild type cells and in cells silenced for α7 and assessed effects of silencing on myogenic differentiation.
The authors filtered genes with low and poorly measured expression, and with low expression variation, retaining 15, 293 genes.
We also measured expression and compared upregulated and downregulated genes by real-time PCR.
To evaluate the ability of 27-OH and 24-OH to modulate α-secretase, we measured expression and protein levels of the main enzyme with α-secretase activity in neurons, that is, ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10).
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Tools and software for measuring expression and codon and amino acid usage in the various datasets are described in File S3.
High Cq values indicate that gene expression levels are quite low, and this in turn causes high variation in accurately measuring expression and reduces the reproducibility of results [ 90– 92].
For each target gene we had 59 iterations, and we calculated the Pearson correlation coefficient (PCC) between the measured expression values and the predicted ones.
We measured expression levels and subcellular localization of HDGF protein in 122 archived paraffin-embedded EC samples using immunohistochemical staining.
In order to determine if mTORC2 is activated in ΔF508 cell41o- celysatestes, we measured expression of mTOR and phosphorylation of the mTOR protein at serine 2481.
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