Therefore, to test for any confounding effect of age with environmental change, we performed a complementary experiment by measuring egg size and clutch size produced by 20 isolated individuals raised at two contrasted rations (low food and ad libitum food), for each clone and over four months.
We could not directly measure egg size for crayfish used in the experiment because removal of eggs from females causes egg mortality.
Resource-related maternal effects may be difficult to measure via egg size alone [ 12].
When measuring egg noodles using measuring cups, 2 oz.
There was, however, no significant relationship between number of eggs measured in a clutch and any of the measures of egg size (length, maximum width, and estimated volume; | rs| ≤ 0.06, N = 668 clutches, all P > 0.14).
This method provides a highly reliable measure of egg size in P. aegeria because there is a strong correlation between egg area and egg mass in this species [ 49].
This method is a highly reliable measure of egg size in P. aegeria as there is a strong correlation between egg area and egg mass in this species (cf. Bauerfeind and Fischer 2008).
Therefore, we likely did not measure the true egg size of our formalin preserved eggs.
Mean initial egg size (measured as egg diameter, mm) differed significantly among demes (one-way ANOVA: F3, 116 = 7.13, P = 0.0002, R= 0.123).
Most measured traits such as egg size, the length of adult aestivation, and egg diapause (at 6°C) showed intermediate values with some maternal influence for both hybrid lineages in the F1 or F2 generations when compared to the parental biotypes.
