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Prior research suggests that the ratio between the ultrasound measured diameter of the IVC and the aorta correlates with the level of dehydration in children with good inter-rater reliability [17],[17].
DBH = D_{s} left( {frac{130}{{H_{s} }}} right)^{a} exp left( {b left( {130 - H_{s} } right)} right), (1 where DBH is estimated tree diameter at breast height in cm, Ds is measured diameter of stump in cm and Hs is the height above ground of the Ds measurement in cm.
We then applied the Abercrombie formula (Abercrombie 1946), which uses the average measured diameter of the germ cell nuclei and the thickness of sections to correct for any double counting due to single cells appearing in two successive sections.
Fluorescent beads with a diameter of 0.20 μm (i.e. similar to the 0.3 μm diameter of insulin granules in rat beta cells [ 40]) had a measured diameter of 0.50 ± 0.02 μm (n = 12).
We then used the Abercrombie formula (Abercrombie 1946), which uses the average measured diameter of the germ cell nuclei (D) and the thickness of sections (S) to correct for any double counting due to single cells appearing in two successive sections, to obtain the true count (TC): TC = CC × S/(S+ D).
Besides, we also measured diameter of mitochondrial tubules in EM image (Supplementary information, Figure S14), we measured the diameters of mitochondrial body and tube along the direction of tube (yellow line), and the maximum diameters (red and green arrows) were taken as final diameters.
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Open image in new window Figure 4 Measured diameters of apples during a period of 12 weeks.
(A ) Measured diameters of the tubes observed by TEM.
We measured diameters of the caput and cauda epididymal tubules (35 tubules/section from five samples of each group) using ImageScope viewer software (Version 9.1.19.1569; Aperio).
Antibacterial activity was determined by measuring diameter of the zone of inhibition (ZOI) surrounding discs.
Evidence of cardiomyocyte hypertrophy was determined by measuring diameter of cardiomyocytes following histopathological processing of the harvested hearts.
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