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Moreover, we measured cell viability upon treatment with ouabain and other apoptosis inducers.
QL, CZ, XL, MYT, HJL and CL measured cell viability and virus replication in biosafety level 3 facilities.
To determine their potential toxicity to human cells, we measured cell viability and ROS formation in two human cell lines using CoCl2 for comparison.
Thus, our results indicated that knockdown of ATP1A1 specifically sensitized cells to aurilide B. Next, to determine whether a Na+/K+ ATPase inhibitor could mimic the effect of ATP1A1 knockdown on aurilide B sensitivity, we measured cell viability upon treatment with both aurilide B and ouabain, a well-known Na+/K+ ATPase inhibitor that acts by targeting α-subunits12, 13.
Therefore, we are interested to investigate whether normal cells and cancer cells differentially respond to AgNPs, to examine the effect of both the cells which were treated with various concentrations of AgNPs and measured cell viability.
To illustrate this point, even though the biological assay data for the Scripps and NCGC compounds was actually obtained from experiments using the same cell line (Jurkat) and measured cell viability determined by ATP concentration, the sensitivity of the NCGC model (0.32) is much lower than the sensitivity of the Scripps IC50 model (0.57).
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We also implemented assays to quantify drug response by measuring cell viability after staining of live organoids with specific dyes followed by imaging.
The pro-drug concept was tested in vitro by measuring cell viability and 6 formation in SH-SY5Y cells subjected to oxygen glucose deprivation (OGD).
This assay simultaneously measures cell viability and cytotoxicity, which is independent on total cell number.
CellTiter-Glo is a luminescent assay used to measure cell viability by ATP level.
Finally, a CCK-8 assay was utilized to measure cell viability.
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