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At regular intervals after elutriation, we measured cell size and the fraction of budded cells.
To investigate this, we measured cell size across the studied cell lines using the forward scattering channel of the flow cytometer.
To quantify these differences, we measured cell size with a fluorescence-activated cell sorter (FACS); the forward-angle light scatter (FSC) parameter correlates with cell diameter [7].
We analyzed these images using an automated analysis program that identified cells expressing DsRed, determined if they were myocytes based on the intensity of anti-α-actinin staining, and then measured cell size.
We measured cell size distributions and fitted them to a log normal distribution to determine the mode, as a measure of the dominant cell size, and standard deviation, which we take as an approximation for noisiness in polarization dynamics.
To test whether ddTpp1 affects the autophagic response of cells, we cultured cells in ASM and measured cell size and cell viability as a function of time.
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Briefly, in adipocyte suspensions, we measured cell sizes by direct microscopy and the mean adipocyte diameter was calculated from measurements of 100 cells.
Using chemical stains they developed to measure cell size and the number of nuclei, the investigators determined that the heart expansion was from hypertrophy, not formation of new cells.
To measure cell size in parallel on a genome-wide scale, we subjected pools of the haploid deletion collection grown in rich media to centrifugal elutriation.
This is further investigated by measuring cell size.
For measuring cell size at septation, cells without cdr2-GFP were analyzed.
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measured cell cycle
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measured cell area
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measured cell viability
measured drop size
measured particle size
measured cell adhesion
measured cell number
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measured cell potential
measured body size
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