It seems that most of controversies in developmental hematology arise from the excessive reliance on measuring cell potentials rather than cells fates.
We have measured cell recovery, viability, phenotype, proliferative potential, and differentiation into mesenchymal (adipogenic, osteogenic, chondrogenic) and nonmesenchymal (neuron-like cells) lineages.
To measure cell invasion potential, matrigel invasion assays were performed as described previously [ 7, 25] using Transwells (BD biosciences, San Diego, CA, USA) with 8-μm pore size filters.
For various HDACIs, we determined an optimal concentration in terms of both their cellular toxicity and their HIV-1 reactivation potential by measuring cell viability (Figure S1 and Text S1) and induction of p24 production in HDACI-treated U1 cells (Figure 1).
Potential experiments may measure cell volume post hypo-osmotic stress with and without specific protein blockers.
All tests were monitored by measuring cell and ferrous iron concentrations, pH and redox potential.
This assay measures the stem cell potential in growth-inhibitory "selection" primary cultures (LC1) by determining the capacity of cells at the end of LC1 to repopulate growth-permissive "expansion" secondary cultures (LC2) and evaluating the kinetics of this repopulation.
To monitor the corrosion process, AE measurement was applied, along with measuring half-cell potentials and polarization resistances at the surface of the RC specimen.
Multivariate linear regression was used to examine the association between hormones (independent variable) and natural log-transformed measures of stem cell potential (dependent variable), adjusting for maternal and neonatal characteristics (mother's age, race of parents, number of live births, gestation duration, baby's gender and birth weight, delivery time, and study site).
