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Next we measured cell area of adherent cells at 20 hours.
To clarify again whether the spinal cord phenotype resulted from enlarged neuronal cells bodies or an increased number of neurons, we measured cell area (Fig. 4H) and counted the number of cell nuclei in the spinal cord (Fig. 4J) of Tg β-actin:mGFP) Tg β-actin wild-type and mGFPntransgenic
We measured cell area and found that w adherent hemocytes have a mean cell area of 224 µm while eater mutant adherent hemocytes have a mean cell area of 129 µm (Fig. 3E), consistent with a possible requirement of Eater in cell adhesion.
This cell behavior was consistent and repeatable over 8 hours of media pH changes, resulting in oscillations of measured cell area that initiated within minutes.
To do this, we use the measured cell area heterogeneity (n = 700) and mean dose at death, nth (indicated in Figure 7 A) to calculate the distribution in time of death, td, using a reformulation of eq 2 : 3 Cumulative integration of the td distribution across a time interval t then provides a predicted value of the % cell death at t.
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The details on all measured cell areas in different tissues can be found in table 2. Figure 2 depicts the results for the individual observers and for the automated cell counting algorithm.
In a similar procedure to the one used to measure cell area oscillations, we computed the trend in myosin fluorescence intensity minima via a moving boxcar average.
The same configuration was used to measure cell areas in all experimental groups.
Morphometric analysis of adherent C2C12 cells was carried out by measuring cell areas and the numbers and lengths of individual peripheral fascin or F-actin bundles from calibrated digital images using the software Improvision Openlab (Perkin Elmer, Waltham, MA, USA).
A marker line expressing GFP in both layers of the outer integument enabled us to measure cell areas in the two outer integument cell layers of developing Arabidopsis wild type seeds and can be used for a high-throughput screen for mutants with altered cell division patterns in the integument.
We measured cell body area in cells isolated from different larval stages and found that cells isolated from L4 stage larvae were on average 2.7-fold larger than L1 derived cells (Figure 5B).
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