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Target cell lysis is typically measured by quantification of released endogenous enzymes, e.g., lactate dehydrogenase, or measurement of released exogenous labels, e.g., 51Cr, europium or calcein.
While CETSA relies on an irreversible thermal aggregation of the target protein, and is therefore not a system at equilibrium, we attempted to derive an apparent intracellular binding affinity, measured by quantification of target engagement within live cells.
Basal cells in TCDD- and DMSO-treated organotypic cultures show no differences in proliferation as measured by quantification of 5-bromo-2′-deoxyuridine (BrdU -positive nuclei.
The calcium deposition was measured by quantification of o-cresolphthalein complexone after 3 and 4 weeks.
Liver parasite burden was measured by quantification of parasite-specific 18S rRNA in total liver RNA by quantitative-competitive reverse transcription-PCR and by an automated 5' exonuclease PCR.
The mineralization of calcium phosphate by MG 63 cells seeded on TCP and scaffolds was measured by quantification of o-cresolphthalein complexone.
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According to the ex vivo experiment, the activity of CYP3A was measured by the quantification of 1-hydroxymidazolam, which was the metabolite from CYP3A probe substrate, midazolam.
After 21 days of culture, SMC elastin production, measured by immunofluorescence quantification, showed HA-o to increase cellular deposition of elastic matrix twofold relative to HA-o-free GM HA gels.
(b) STEM photograph and the chemical composition were measured by point quantification.
The chemical composition of nanoparticles at the particle level was extensively measured by point quantification in STEM-EDS analysis.
Figure 7A and B shows that Syt VII levels on the plasma membrane and cell surface are significantly lower in NC9 treated cells and decreased down to 29% of controls as measured by immunofluoresence quantification (Fig. 7B).
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