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The cell trajectories and velocity were measured by manually tracking the leading edge of each cell/protrusion using the Manual Tracking plugin of ImageJ.
Velocity-time integral (VTI) was measured by manually tracing the flow envelope for each image (Fig. 1).
As an MRI parameter, mean ADC (ADCmean) for head and neck cancer was measured by manually drawing a VOI on the ADC map using monoexponential ADC calcucalation method.
The mean count values of the reference tissues were measured by manually drawing VOIs in the inside of the vertebral body such that the borders coincided with the vertebral cortical bone shell, as reported previously [17].
Cell area and circularity were measured by manually rendering the cells in the phase images.
Briefly, fluorescence intensity of relevant structures from unaltered unsaturated confocal maximum intensity projections was measured by manually selecting the region of interest in imageJ and measuring mean pixel intensity of that region.
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The test began immediately after each subject was placed in the corner of the apparatus and latency to feed the pellet was measured by counting manually.
In addition, ESV was manually measured by dipping a relaxed inner membrane unit into a water path.
Hence, we set out to compare the values manually measured by Andrews with those, based on the same anatomical reference points and planes, obtained using digital technology to determine whether Andrews' values are still relevant or whether significant differences indicate that digital measurements provide us with better reference values.
All LV structures were manually measured by the same observer as previously described [ 26].
Conventionally, the IMT is manually measured by the trained operator from the US scan images.
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