The DOTA substitution level of the Fab fragments (chelators/molecule) was measured by labeling a 10 μL aliquot of the unpurified conjugation reaction with 111In, then determining the proportion of 111In-DOTA-Fab vs. free 111In-DOTA by ITLC-SG and multiplying this fraction by the molar ratio used in the reaction [26].
Cells cotransfected with receptor and either PCDNA3.1 or RACK1 were treated with 1 mM carbachol for 8 hours and total receptor was measured by labeling with the membrane permeable radioligand [3H]QNB.
However, the number of circulating BMPCs, as measured by labeling cultured peripheral blood MNCs with acLDL/isolectin B4 [42], was not significantly reduced in the presence of cyclopamine (Figure S6).
G2 was measured by labeling animals with EdU (via feeding) and calculating the fraction of M-phase cells (mitotic figures) that were EdU+ over time (equation 1).
Steady-state isotopic glycolytic metabolism was measured by labeling cells with [C] or [C]glucose and quantifying both nonisotopic and isotopic incorporation into glycolytic intermediates.
The percentage of apoptotic cells in tumour sections may be measured by labeling fragmented DNA breaks and calculating the apoptotic index (AI) using the TUNEL (terminal transferase uridyl nick-end labeling) assay [ 8].
However, in most of these studies, neurogenesis was measured by labeling the newborn cells with a thymidine analogue such as bromodeoxyuridine (BrdU) or by expression of markers for proliferation and immature cells.
Blood erythrocyte self-TG2-autoantibody complexes are captured from the haemolyzed sample by TG2-binding proteins onto a solid phase and the presence of autoantibodies is measured by labeled antihuman IgA [ 28].
In the second approach, the growth rate of L. mexicana amastigotes in inflammatory lesions in susceptible BALB/c mice was measured by labeling infected mice with heavy water (H 2O).
Cell migration for 20 h toward the chemoattractant EGM-2MV was measured by labeling the migrated cells with Calcein AM Fluorescent Dye (BD Biosciences) according to the manufacturer's instructions.
