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The delay of each path is then measured by adding the delays over its links.
The statistical segment length (Lk) for each copolymer with or without NaCl was measured by adding the intrinsic statistical segment length (L0) to the electrostatic statistical segment length (Le) [7, 8, 47].
After washing away unbound ACE, plate-bound ACE activity was measured by adding the ACE substrate (Hip-His-Leu or Z-Phe-His-Leu) directly into wells [30].
At the time of activation, the pro-TPPI was incubated with 25 µM NBS, 10 mM CaCl2 or 10 mM EGTA for 15 min. Following this preincubation, the pro-TPPI was activated by autocatalysis in 50 mM ammonium formate buffer, pH 3.5 for 5 min at 37°C, and finally TPPI activity was measured by adding the substrate GFFL-AFC.
Threshold potentials for VGSC activation were measured by adding the negative voltage pulses (5 ms in duration and 2 mV in each of steps), and refractory periods for VGSC reactivation were measured by changing inter-pulse intervals in 4∼10 ms. Data were analyzed if the recorded neurons had resting membrane potentials negatively more than −65 mV.
Caspase activity was measured by adding the diluted caspase substrate (DEVD-R110) at 37°C for 2 hours.
Similar(48)
The sizes of the gaps range from 0.6 kb to 2.4 Mb, measured by adding up the sizes of the un-matched optical fragments at the gap locations.
In regard to the explanatory variable, the overall performance of mediation is measured by adding up the cases mediated before and after they were admitted by arbitral institutes (see Eq. 1).
Note that the total computational cost has to be measured by adding up the costs incurred at all resolution levels.
The substitution dynamics of CpG-containing codons are measured by adding parameters to the CNFGTR baseline.
After 24 h, caspase-3/7 activity of the cells was then measured by adding an aliquot of the Homogeneous Caspase-3/7 reagent to each well, shaking the plate for 1 h at room temperature, and measuring the fluorescence intensity by a fluorescent plate reader (Wallac Co., Turku, Finland).
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