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By means of phase locking techniques, any round-trip phase delay change gives a measurement of a change in the SC distance (measured as light transit time), due to incoming GWs.
For Symbiodinium culture Z1, inactivation (I) of PS II (measured as light adapted quantum yields) was greater than 95% (mean I (Z1 ΔF/Fm') = 0.968±0.016) following exposure to P1 supernatant for 10 min in two independent experiments, and total PS II inactivation resulted after 20 min (Fig. 1A).
Following cell lysis, luciferase reporter activity was assessed in 20 µl of cell extract which was mixed with 100 µl of the luciferase assay reagent, and firefly luciferase activity measured as light output (10 s) in a luminometer (Berthold Detection Systems, Huntsville, Al).
(B ) Food intake (N = 3) measured as light absorbance (a. u., arbitrary units) of ingested sulforhodamine B added to a standard diet (sd) or morinda diet (md).
After transfection, cells were lysed with 1 × passive lysis buffer (Promega, Madison, WI, USA) and luciferase activities were measured as light emission after addition of Renilla and Firefly luciferase substracts using a luminometer (Mithras, Berthold Technologies, Bad Wildbad, Germany).
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The isoluminol conjugate is oxidized when sodium hydroxide solution and peroxide solutions (triggers) are added to the cuvette, and the flash of light produced from this reaction is measured as relative light units (RLUs) by the BIO-FLASH® optical system.
Our results confirm that subjects not only have poorer perceived sleep quality, but also suffer from sleep disruption, measured as more light sleep, longer REM latency and less REM sleep, with exposure to simulated ICU noise and light levels.
On the other hand, a cool dwarf star with a surface temperature of 3,000 K has an energy maximum on a wavelength scale at 10000 angstroms in the far-infrared, and most of its energy cannot therefore be measured as visible light.
The luciferase activity per well was measured as relative light units (RLU).
The reaction on BIO-FLASH is measured as relative light units (RLUs) by the BIO-FLASH optical system.
Luminescence was measured as relative light units (RLU), taking the reading of luciferase assay substrate alone and then with lysate in GlowMax 20/20 luminometer (Promega, Madison, USA).
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