Potential NH3 and CH4 emissions were measured in reconstituted slurry samples over 11 and 100 days, respectively.
We demonstrate how conformational dynamics of a G-protein-coupled receptor can be measured in reconstituted systems.
The extracts were dried down and reconstituted in 1.0 ml buffer and measured by radioimmunoassay Kit (provided by Neurobiology Department of The Second Military Medical University).
The supernatant was evaporated to dryness, and reconstituted to same volume, then absorbance was measured after 15 min.
An application of the new test procedures involved conducting several experimental tests on undisturbed and reconstituted soil samples highlighted the simplicity of this apparatus in measuring thermal conductivity of soil under different conditions.
Indeed, the extent of quenching of fluorescence was shown to be FtsW concentration dependent, as measured from vesicles reconstituted with different amounts of FtsW.
Dengue virus IgM antibodies measured in serum reconstituted from blood spots on filter papers were lower than in serum, in particular in the acute phase of disease.
This agrees well with the pH dependence of lactose efflux measured with proteoliposomes reconstituted with purified LacY, where a functional group or proton binding site with a p K of 8.3 must be deprotonated for LacY to catalyze efflux at maximum rates (31).
After adding triethanolamine solution and Assay Cocktail [a mixture of MES buffer (11.25 ml), reconstituted cofactor mixture (0.45 ml), reconstituted enzyme mixture (2.1 ml), water (2.3 ml), and reconstituted DTNB (0.45 ml)], total GSH in each of the deproteinated samples was measured at 405 nm.
The supernatant was evaporated and reconstituted with assay buffer, and then LTC4 was purified through a Sep-Pak column (Waters Associates, Milford, MA, USA) and measured using a specific immunoassay (Cayman) according to the manufacturer's instructions.
