The fluorescent signals associated with calcium-free and calcium-bound Fura-2 were measured by alternatively exciting the slices at 340 and 380 nm using a Leica DM fluorescent microscope equipped with a rapid shutter wheel.
Animals had to have dependent variable reliabilities equal to or above this cut off to be considered for analysis as typically they have either not been measured or alternatively progeny tested for the trait.
Fura-2 excitation ratios were measured by alternatively exciting the dye at 340 and 380 nm, at a frequency of ∼1 image pair every 4 s and collecting emission at 510 nm using Flexstation III (Molecular Devices) equipped with SoftMax Pro 5 software (Molecular Devices).
Following the notation in ([Richter 2013]), we will call the star-generalized surface measure alternatively the Ea,p-generalized surface measure if K is a p-generalized ellipsoid with axes of lengths 2a i,i=1,…,n.
More formally, a fuzzy measure (alternatively called a capacity in the literature) is defined as follows: (Fuzzy measure): Let N = { 1, 2, …, n } be the index set of n criteria.
The validity of this algorithm for spatial field reconstruction was benchmarked by theoretical considerations as well as comparison of the calculated with alternatively measured data.
The absorbance of the reaction product was alternatively measured in the presence of excess of either NBD Cl or SER.
The fluorescence intensity of the reaction product was alternatively measured in the presence of excess of either FMOC-Cl or SER A plot of log fluorescence versus log [FMOC-Cl] and log [SER] gave two straight lines, the values of the slopes are 0.707 and 0.985 respectively(Figure 5).
