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To test this, we measured adhesion force and Young's modulus across the whole differentiation process to further support the changes in function and cell surface ultrastructure.
To analyze the effects of fixative on cellular mechanical properties, we firstly measured adhesion force of fixed and unfixed lymphocytes (Fig. 3a, b).
Figure 2 shows three representative force displacement curves obtained by AFM measurement between rat anti-human IgG monolayer formed on thiol-based SAM substrate and (1) original bare tip, (2) blocked tip prepared as described in "Specificity of the Measured Adhesion Force", (3) tip coated with human IgG.
The surface wettability of rectangular and sinusoidal nanostructures was measured and compared with the measured adhesion force and the number of particles remaining after centrifugal detachment tests.
The measured adhesion force (352.37 ± 11.71 pN) on the membrane surface of the mature BMDCs was much bigger than that (70.37 ± 4.55 pN) of the immature BMDCs (n = 10; P < 0.01).
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on the observed wetting behavior was established, and in some cases quantitative agreement between measured contact angles and measured adhesion forces was obtained.
Measured adhesion forces scaled inversely with solvent polarity, with greatest adhesion observed in the least polar solvent, methanol.
Solvent polarity affected the elasticity of the bacterium, the conformation of the cell surface biopolymers, the height of the surface biopolymers, and measured adhesion forces between the bacterium and silicon nitride.
(a) Distribution histograms of measured adhesion forces in PBS.
(c) Distribution histograms of measured adhesion forces in tylosin solution (10 mM).
(d) Distribution histograms of measured adhesion forces in levofloxacin solution (10 mM).
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