In any RNA-seq sample, any transcript for which its MMSEQ posterior median TPM was lower than 0.01 was set to 0.01 (which we thus used as the minimal measureable expression level and therefore avoid taking the logarithm of 0).
By the blastocyst stage of human development, measureable differences in the expression of these proteins as well as in the autosomally encoded DNA methyltransferases DNMT3 A and B are evident.
It has been reported that relatively high concentrations of LH increases proliferation and invasiveness of SKOV-3 cells [ 46, 47], which is somewhat surprising in view of the absence of measureable LHR expression in these cells [ 14, 19, 20].
Immunoblot and RT-PCR analyses indicated that A2780.140 and MCF-7.MR cell lines have similar high expression of BCRP, despite MCF-7wt cells expressing low but measureable levels of BCRP, as previously noted (Robey et al, 2007), and A2780wt cells having no detectable BCRP expression.
The expression of pY705-STAT3 was measured in tumor samples in this study, but almost no pY705-STAT3 expression was detected, and no clear decrease after administration was found in the measureable samples.
Butanoyl-CoA dehydrogenase is known to be very oxygen sensitive and yet expression of bcd and etfAB under aerobic conditions led to measureable butanol production in E. coli (Atsumi et al. 2008).
Indeed, closer evaluation identified a pattern whereby expression of the transgene altered normal developmental processes in subtle, but measureable, ways that were not immediately apparent.
Gene expression changes, altered metabolite concentrations, or other biochemical markers are measureable indicators of cellular and biochemical changes.
In these cases, individuals with below LOD measurements were included I the exposure group of individuals with the lowest measureable quantities of the PCB/OCP compound.
Measurements of intracellular acyl-CoAs in induced cultures of strains from Strain Set II revealed detectable quantities of 3HB-CoA, showing that expression levels of hbd in Strain Set I had been insufficient for measureable intracellular conversion of AcCoA to 3HB-CoA, and that intracellular NADH levels were sufficient for production of 3HB-CoA.
