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In preliminary dose escalation experiments, we did not detect a measureable effect of Herceptin® on proliferation in SKBr3 cells at any dose or exposure times tested (1 10 nM, 12 72 hours, data not shown).
In vitro, there is no measureable effect of calcium on BiP oligomerization, in either the ATP state or ADP state or in the presence of the J-protein ERdj3 or the exchange factor GRP170 (not shown).
87 88 The lack of a measureable effect of improved hand hygiene on MSSA infections might seem paradoxical but can be partly explained by the fact that MSSA infections are much more likely to be of endogenous origin, whereas MRSA is more often linked to nosocomial cross transmission.
In vitro, there is no measureable effect of calcium on BiP oligomerization, in either the ATP state or ADP state or in the presence of ERdJ3 or the exchange factor GRP170 (not shown), yet in vivo, ER calcium depletion promotes such oligomerization.
Reviewer #3 The lack of any measureable effect of ISRIB on baseline translation is surprising as there is evidence for basal activity of the ISR: For example PERK_KO, eIF2a_S51A and ATF4_KO cells all share a strong baseline requirement for amino acid supplementation (likely indicating that the ISR contributes to baseline ATF4-mediated gene expression).
However, the reductive shift, as measured by decreased fluorescence lifetime of ER-localized roGFPiE, correlated well with the extent of PDI1A knockdown across the six different shRNA constructs used, consistent with a measureable effect of attenuated PDI1A availability on ER redox poise.
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Fortunately initial volume did not have a measureable effect on any of the detection assays with OP swabs.
By monitoring the lifetimes of the A and B states, which effectively provides the dwell times of the labeled and unlabeled heads, respectively, we demonstrate that the 20 nm gold particle attached to the N-terminus of myosin has no measureable effect on the kinetics of stepping.
Examination of graphene integrated with two different MRR devices, one with radii of curvature r = 10 μm and the other with r = 20 μm, indicates that the device geometry has no measureable effect on the level of doping.
Furthermore, a large number of proteins are differentially expressed in the heart as a consequence of Txnip deletion, despite no measureable effect on thioredoxin activity under baseline conditions.
This dose of OLZ had no measureable effect on whole body oxygen consumption, V o 2, when injected into either brain region.
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