Sentence examples for measure transfection efficiency from inspiring English sources

To measure transfection efficiency, HBE and HELMF cells were transfected with a CMV-driven GFP reporter gene, and the number of GFP fluorescing cells was expressed as a proportion of DRAQ-5-stained nuclei.

Cells were co-transfected with pEGFP-C3 so as to measure transfection efficiency using GFP expression.

AIP1-luciferase reporter construct plasmid DNA was co-transfected with pRL-TK Renilla luciferase expressing vector to measure transfection efficiency (Promega).

In addition to the 5 µg plasmid DNA, 100 ng of GFP containing plasmid was added in order to measure transfection efficiency.

Alexa 568 labeled negative control siRNA (Qiagen) was used to measure transfection efficiency.

To measure transfection efficiency, cells were electroporated with EGFP-pCIpA102 mRNA [ 14] encoding the enhanced green fluorescence protein and analysed by flow cytometry (FACSCalibur, Becton Dickinson).

A FAM-labeled negative control LNA oligonucleotide (Ambion, # AM 17012) was transfected in the same conditions as those used for the unlabeled LNA molecules, and used for measuring transfection efficiency by fluorescence microscopy at 48 hours after transfection.

Again, we measured transfection efficiency in each experiment (Fig 2D).

The respective fitted value to each measured transfection efficiency was also deduced from the established model.

FFL mRNA levels were normalized to RL, as a measure of transfection efficiency and to β-actin, as a control for the amount of mRNA.

Cells were transfected with a MUC4 promoter-luciferase reporter construct (wild-type or mutated) [9], [10] along with an ELF5 expression vector and a β-galactosidase expression construct that was used as a measure of transfection efficiency.