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The resulting polychromatic and multiplexed imaging assay enabled us to measure the secretion of mycobacterial effectors inside infected host cells.
We next used ELISA to measure the secretion of cytokine and chemokine proteins from ud- and wd-NHBE cell culture supernatants infected by influenza H1N1 and H5N1 viruses.
Unfortunately, assays for GDF5 were not available to measure the secretion from decidualized cells.
Therefore, in our assay, we only measure the secretion of MUC5AC contained in the post-Golgi secretory carriers.
We have established a quantitative assay to measure the secretion of MUC5AC from a human goblet cell line.
To measure the secretion of CLP-induced cytokines or chemokines in peritoneal lavage fluids or blood, mice were given peptides 2 and 14 h after CLP.
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The vaccine- specific cellular immune response in mice was determined using ELISPOT assay measuring the secretion of IFN-γ by splenocytes after stimulation with different peptides pools.
Therefore, we transfected the pMAC/PS-scFv into CHO cells, and after 24 hours, we measured the secretion of scFv by dot immunoblotting.
We measured the secretion of IL-8 by DTT-treated Del-N35TCTP, BMOE or BM PEO 3-treated RrTCTP, and BM PEO 3-treated-tRrTCTP DTT-pretreandd-Del-N35TCTP (Figure 5BMOE
This loss-of-function effect was confirmed by measuring the secretion of endogenous mediators in supernatants of HEK 293T cells (n = 3, p<0.0001; Fig. 4C).
Thus, the clear loss-of-function effect of S183I RIG-I SNP was confirmed by measuring the secretion of IL-8 (n = 3 p<0.0001; Fig. 3D) and RANTES (not illustrated) in the supernatants of stimulated HEK 293T cells.
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