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A modified version of a previously described assay for the intracellular conversion of nitro blue tetrazolium (NBT) to formazan by superoxide anion was used to measure the generation of reactive oxygen species (Vrablic, Albright, Craciunescu, Salganik, & Zeisel, 2001).
All of these features make use of DR-restricted T cell hybridomas an excellent means to measure the generation and presentation of peptide:MHC complexes in isolation from effects on human CD4+ T cells.
The following assay was developed to measure the generation of F2,6BP by relatively intact pure nuclei.
Figure 1b shows an example of how intermolecular FRET can be use to measure the generation of diacylglycerol at any membrane of interest.
Luminol-dependent chemiluminescence was used to measure the generation of both extracellular and intracellular ROS by neutrophils upon HP-NAP stimulation.
These colonies expressed the fluorescent reporter at or close to its equilibrium concentration and the rate of fluorescence increase was used to measure the generation time, τgrowth.
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To measure the generations of specific ROS, two probes were used respectively.
This task measures the generation of a Unique SMILES string.
Adequacy measures the generation and transmission capacities of the system under static conditions, without considering system disturbances [3].
PLD activity was determined by measuring the generation of 3H-labeled choline from [choline-methyl-3H]dipalmitoyl-PC (NEN Life Science Products, Boston, MA) (phosphohydrolase activity) and 3H-labeled phosphatidyl butanol (PBut) (transphosphatidylation activity) as previously described [40], [41].
Since peroxynitrite is formed by the interaction of superoxide and NO, we next measured the generation of the latter two substances along with [Ca2+]i mobilization by measuring dihydroethidium (DHE, a fluorescent indicator of superoxide), and diaminofluorescein-2 diacetate (DAF-2, an indicator of NO) fluorescence, respectively.
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