Since its modern beginnings [78,307 310], metabolomics is significantly seen as an analytical science, in that it depends on our ability to measure sensitively, precisely and accurately the concentrations of a multitude of chemically diverse metabolites.
In interviews with hospital staff the importance of introducing the measure sensitively was emphasised, as was the need to be clear about how the results would be used, as this would help to ensure staff co-operation.
These measurements illustrate that the ERα bioaffinity of the phytoestrogen coumestrol could be measured sensitively and efficiently with the new E2-F based HRS ERα FP system, which is not possible due to the occurrence of autofluorescence using the coumestrol-based ERα HRS system developed by Oosterkamp et al. [ 10].
FABP can be measured sensitively in both plasma and urine using an enzyme-linked immunosorbent assay (ELISA).
Our results indicate significant differences in the responses to both the EQ-5D-3L™ descoretive score and VAS score between baseline pre-injury status, and one-month post-injury indicating that both measures sensitively differentiate injured from non-injured states in the paediatric population.
Second, activity of SEAP can be measured quickly, sensitively, and quantitatively with conventional chemiluminescent systems (Kasai et al. 2005, 2004).
In particular, PDS is a very powerful technique for measuring very sensitively the optical absorption due to defect states within the bandgap of the semiconducting oxides.
Our field measurements showed that the phloem turgor pressure probe can sensitively measure the real-time variation of phloem turgor pressure in H. brasiliensis but the calculation of phloem turgor pressure with xylem tension, xylem sap osmotic potential and phloem sap osmotic potential will under-estimate it.
To sensitively measure cDNA in each reaction, Quanti-iT Oligreen ssDNA reagent (Molecular Probes, CA) was used to measure and aliquot single-stranded cDNA in combination with a fluorometer (Turner Biosystems).
It confirmed the newly developed PTPP could sensitively measure the quick change of the H. brasiliensis phloem turgor pressure which is an indication of the latex flow and phloem water relationship after tapping.
It could sensitively measure the rapid alteration of phloem turgor pressure for several hours (Figures 3, 4, 5, 6 and 7; Additional files 1, 2, 3, 4 and 5), and in accordance with tapping and daily transpiration induced trunk diameter (Gooding 1952; Luštinec et al. 1969) and bark contraction (Southorn 1967).
