Sentence examples for measure receptor from inspiring English sources

Characterisation of receptors can involve either assessment of their ability to bind ligands or measure receptor activation as a result of agonist or inverse agonist interactions.

To measure receptor activator of nuclear factor κB ligand (RANKL) protein levels from the cell supernatants after 48 h culture and bone marrow supernatants after flushing the bone marrow with 500 μL of PBS, RANKL Quantikine ELISA kit (R&D Systems) was used and the assay was performed in duplicate according to manufacturer's instructions.

Exploiting these observations, we have designed and validated an assay that allows us to measure receptor occupancy in vitro on monkey and human peripheral blood leukocytes and ex vivo in whole blood from monkeys dosed with small molecule LFA-1 antagonists.

Working with ZEISS instruments and Software, I experienced a full range of imaging techniques - to measure receptor signalling (fluorescent dyes that sense intracellular calcium) and to image and co-localise receptors, fluorescent ligands and markers for different intracellular compartments.

Consequently, there is an urgent need to develop alternative methods to measure receptor activation.

Intracellular Ca2+ mobilization provides a rapid, sensitive and real-time assay to measure receptor desensitization [28].

We used I-EGF uptake to measure receptor-mediated endocytosis in KB-3-1 and KB-CP20 cells.

Examples of applications of PET imaging in neuroscience include studies of neuroreceptor/neurotransmitter levels in neuropsychiatric diseases (e.g., measuring receptor expression in schizophrenia) and of misfolded protein levels in neurodegenerative diseases (e.g., beta amyloid and tau deposits in Alzheimer's disease).

The activity of MdOS against HER-2 was followed by measuring receptor autophosphorylation in naturally HER-2-overexpressing SK-OV-3 cells and a newly generated NIH-3T3 cells lines stably expressing HER-2 (NIH-3T3/neu cells).

In that context, measuring receptor activation signatures constitutes an integral part of an overall diagnostic strategy aimed at identifying potentially responsive patients, stratifying patients for clinical trials and monitoring therapeutic responses to a given drug.

To validate that our measured receptor antigens were in the membrane, and not the cell interior, we monitored our fixation conditions for absence from accidental plasma membrane disruption.