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Melamine contamination became a major scandal in China after it was added to milk to disguise test results that measure protein levels.
The ability to measure protein levels in single cells has become increasingly important in the diagnosis and treatment of cancers, to identify specific molecular markers.
It is used to make plastics and fertilizers, but it is sometimes illegally mixed into food products, including milk, because its high level of nitrogen can help fool tests that measure protein levels.
In this study, targeted proteomics, via selected-reaction monitoring (SRM) mass spectrometry, was used to measure protein levels in Escherichia coli strains engineered to produce the sesquiterpene, amorpha-4,11-diene.
To measure protein levels, actively growing HeLa, SKMEL5 and MCF7 cells were lysed in RIPA buffer and 30 µg of protein was probed for the protein expression.
We used real-time PCR to evaluate IL-15 mRNA levels and FACS as well as ELISA and Western Blot (WB) analysis to measure protein levels and distribution in CaCo-2 cells.
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To measure protein level in ocular tissue, an equal number of mouse retinas and choroids isolated from the different treatment groups were lysed in RIPA buffer (n = 5).
Melamine can also be used to illegally inflate the nutrition value of foods by fooling testers measuring protein levels.
Makers of baby formula, for example, watered down their product, lowering the amount of protein and nutrients, then added melamine, which is cheap and fools tests measuring protein levels.
Peshkin then repeated this experiment, this time measuring protein levels only at the moment of fertilization and excluding the protein quantities throughout development.
We measured protein levels of the enzymatic component of the polycomb repressive complex 2, EZH2, but found no differences in the content of EZH2 in muscle from physically active and inactive men (data not shown).
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measure pollution levels
measure protein dynamics
measure insulin levels
measure hormone levels
measure activity levels
measure serum levels
measure protein carbonyls
measure radon levels
measure acid levels
measure noise levels
measure decibel levels
measure satisfaction levels
measure histamine levels
measure radiation levels
measure estrogen levels
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