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Additionally, immunostaining was used to measure protein expression.
Such techniques are valuable to measure protein expression, solubility, activity, and native posttranslational modification events in heterologous systems.
However, the ability to measure protein expression, produce proteins (usually in recombinant form) and to block receptor ligand interactions remains essential for elucidating the function of the molecules of interest.
Immunohistochemistry was used to measure protein expression of MKP1 and CD31 and to assess cellular apoptosis.
It is majorly used to measure protein expression levels and the intensities of specific bands by using an online software tool (Gelquant.NET).
The term protein expression is however very rarely used in this sense, but tends rather to be used in the context of translation or protein synthesis, which is semantically not correct and further leads to misunderstandings when attempts are made to measure protein expression by two-dimensional gel electrophoresis or liquid chromatography.
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The benefit of using CAI is that it can be computed for any gene with a codon frequency distribution, whereas experimentally measured protein expression or ribosomal loading data are often limited to relatively highly expressed proteins or transcripts.
In these experiments, we measured protein expression levels of all the NADPH oxidase subunits.
As an indicator of Nrf2 activity in vivo, we measured protein expression of NQO1 in lung homogenates.
To determine if ventilator-induced reduction of Ang-1, Ang-2 and Tie2 mRNA also resulted in a reduction of Ang-1, Ang-2 and Tie2 protein, we measured protein expression of these mediators in total lung homogenates.
In addition, we measured protein expression levels in six independent pools of tissue extracts, each containing two of the human or two of the chimpanzee samples, respectively, in two sets of experimental replicates, using ICAT profiling (Materials and Methods).
More suggestions(15)
measure protein volume
measure protein binding
measure protein adsorption
measure protein turnover
measure protein synthesis
measure protein transport
measure protein presence
measure protein translocation
measure protein level
measure protein content
measure protein concentration
measure protein coagulation
measure protein abundance
measure protein kinase
measure receptor expression
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