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High sensitivity of DWP-OCT to measure optical pathlength (op) changes in blood allows application of relatively low-level laser excitation intensities.
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(3) and 4 is written as where By measuring differential optical pathlength (Δop) in blood at two wavelengths normalized by incident excitation light intensities, SaO2 levels can be computed directly.
RI was obtained by taking the ratio between the optical pathlength measured by OCT and the focus shift resulted from translating the focus of an objective lens inside biological tissues [ 1].
The cured polymer's refractive index (n) is 1.41 ± 0.01 in the near infrared (800-1300 nm), determined by OCT optical pathlength measurements of samples with known physical thickness.
The optical thickness L o can be measured from the MPM cross-sectional image and the optical pathlength L p can be measured from the OCT image.
Depth-resolved Photothermal OCT signals corresponding to variation of optical pathlength (op) was measured at five depths in the blood sample (Fig. 2): 1 – the upper air-vessel interface (op = 73 µm), 2 – upper vessel-blood interface (op = 187 µm), 3 – lower blood-vessel interface (op = 572 µm), 4 – vessel-epoxy interface (op = 676 µm), 5 - epoxy-glass slide interface (op = 749 µm).
To measure SaO2 levels in a blood sample, influence of optical pathlength changes in overlying layer(s) must be excluded and requires measurement of differential optical pathlength (Δop) between two longitudinal points.
A phase sensitive (PhS) OCT system (Fig. 1) was used to measure nanometer and sub-nanometer scale changes in optical pathlength in the sample in response to laser excitation.
The optical pathlength L p can be measured from the OCT cross-sectional image.
Phase measurements provided by DWP-OCT are associated with optical pathlength (op) changes in response to dual-wavelength (765 nm and 800 nm) excitation of a blood sample.
The optical pathlength of this layer can be directly acquired by measuring the distance between the two peaks in Fig. 5(b).
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