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Moreover, we studied oxidative stress in three ways: through the measure of reactive oxygen species (ROS) production, through malondialdehyde (MDA) production and also through glutathione regulation.
5,5′-Dithio-bis 2-nitrobenzoic acid) analysis, a measure of reactive sulfhydryl (-SH-) groups, showed that the initial thiol content was not significantly different between the microwave-processed and UHT-treated milks.
As a measure of reactive oxygen species (ROS) we used the ROS-sensitive probe 2,7-dichloro-fluorescein diacetate (H2DCFDA) to monitor AAP induced cellular oxidative stress.
The EndoPAT system (endothelium pulse amplitude tonometry) will be used to give a measure of Reactive Hyperemic Index (RHI), a measure of endothelial function designed to be used in conjunction with FMD measurement of reactive hyperemia.
Luminol-dependent chemiluminescence, a measure of reactive oxygen species (ROS) formation, generated by BAL cells was monitored and the levels of nitric oxide and tumor necrosis factor (TNF -[alpha] produced by macrophages in culTNF -[alpha]termined.
We have previously described the use of the chloromethyl derivative of DCFH (CM-DCFH) to obtain a measure of reactive oxygen and nitrogen species in isolated cultured single skeletal muscle fibers during contractile activity or passive stretching (40, 41).
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Obese individuals have demonstrated markers indicative of oxidative stress, including elevated measures of reactive oxygen species (ROS) [2] and diminished antioxidant defense, which is associated with lower antioxidant enzymes [3].
The RPQ [ 12, 14] includes 23 items that were based on previous teacher-rating measures of reactive aggression and proactive aggression, and on conceptual and theoretical relevance.
To measure levels of reactive oxygen species (ROS) including H2O2 and O2.− generation in infertile men and determine whether sperm quality is correlated with levels of ROS triggered by the exogenous reduced form of β nicotinamide adenine dinucleotide phosphate (NADPH).
We decided to measure inflammation levels by fibrinogen and WBC count without considering the measure of C-reactive protein.
Both the TNBS assay and modified Ellman's test measure the number of reactive functional groups and are a measure of "hapten equivalences" and not hapten density per se.
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