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It is well documented that HFB forms multimeric complexes of quite large size.[ 21] The drive towards multimers increases with concentration, that is, during the drying process, we can expect stronger complex formation compared to the wet gel state.[ 19] In the dry state, we can expect that hydrophobic interactions between the proteins would allow some measure of fluidity in the interactions.
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To our knowledge, this is the first study that investigated the lipophilic index as a measure of FA fluidity in relation to type 2 diabetes risk.
The lipophilic index has been proposed recently as a measure of the FA fluidity of biological samples [ 11, 12].
Only recently, the lipophilic index has been proposed as a measure of overall FA fluidity, which can be easily derived from the FA composition of biological tissues.
In the current study, we aimed to investigate prospectively the lipophilic index as a measure of the FA fluidity of erythrocyte membranes in relation to the incidence of type 2 diabetes.
Another technique used to measure the fluidity of supported lipid bilayers is the lateral migration of charged lipids in the presence of an electric field [ 9, 13, 14].
To measure the fluidity of the membranes in pRBC, we employed fluorescence recovery after photobleaching (FRAP) on small areas of the pEM and PVM (Fig. 4B).
The lipophilic index, which can be derived from the FA profile of blood or tissues, has recently been proposed as a novel measure of cell membrane FA fluidity.
In addition to measuring adhesion, the physiological level of fluidity of our supported membranes makes them ideal for assaying the full T cell activation process.
The reciprocal of the viscosity is called the fluidity, a measure of the ease of flow.
To measure membrane fluidity, 4.3 μl of DPH (736 μM stock solution in ethanol; the concentration was determined by ε350 nm = 88 cm−1 mM−1 in methanol) was added into 400 μl of the cell suspension.
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