Laser-excited fluorescence emitted from individual cells is measured at multiple wavelengths, rapidly, with high sensitivity and accuracy.
The spectral radiation intensities emitted from a one-dimensional McKenna burner is measured at multiple wavelengths using a high-speed mid-infrared spectrometer.
Instead of fitting the values for μ′ s and γ for each wavelength separately, the introduction of a background scattering model as a spectral constraint allows to fit R SF o data measured at multiple wavelengths and multiple fiber diameters simultaneously.
The change in chromophore concentration can be calculated from the change in attenuation spectrum measured at multiple wavelengths using a classical least-squares algorithm if the chromophore extinction spectra are known.
Characterization of small particles ubiquitously involves scattering measurements at multiple wavelengths or at multiple angles.
Furthermore, measurements at multiple wavelengths are utilized simultaneously and absorption coefficients are correlated across these wavelengths within the image reconstruction.
The dual-wavelength unit was also used to measure potential synergistic effects of multiple wavelengths on bacterial and viral inactivation and DNA and RNA damage.
Laser scanning cytometry (LSCM) automatically measures laser excited fluorescence at multiple wavelengths and light scatter from cells on slides that have been treated with one or more fluorescent dyes to rapidly determine multiple cellular constituents and other features of the cells.
By measuring the scattered light at multiple wavelengths within the near-infrared frequency range, one can recover hemoglobin (oxygenated, HbO, or deoxygenated, HbR), water and lipid concentrations as well as the tissue scattering properties.
Multispectral FLIM, as implemented here, directly measures the fluorescence temporal decay at multiple wavelengths by exciting with a high repetition rate pulsed UV laser and recording the emission on a high-speed microchannel plate photomultiplier tube (MCP-PMT) and high speed digitizer.
