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The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of a myriad of clinical assays.
As an extension, we are now profiling CSF from patients with schizophrenia and controls using multiplex immunoassay profiling to simultaneously measure multiple proteins and metabolites.
Furthermore, one substantial advantage is the capacity to measure multiple proteins and develop a normalized metric based on expression of a largely invariant protein.
To this end, we used SELDI-TOF MS that has the possibility to measure multiple proteins simultaneously in a high-throughput fashion.
The Bio-Plex suspension array using specific antibodies and based on the principle of flow cytometry is a high-throughput technology that can measure multiple proteins in low sample volumes.
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Using these assays, 21 single blastosol samples were screened and the 9 tested proteins were detected, thus demonstrating the ability of targeted proteomics to measure multiple protein species deriving from a single embryo blastocoel (Tables EV6 and EV7).
Several recent studies that measured multiple proteins in plasma in patients with AD and controls identified plasma APOE concentration as one of a panel of diagnostic markers for AD [ 6- 9]; however, the extent of additional predictive value beyond APOE genotyping [ 10] remains to be clearly established.
We have recently devised methods of measuring multiple protein kinetics simultaneously[30 32] which could further expand the utility of this method in looking at the pathology of psoriasis or response to treatment.
Now the F.D.A. is saying it will regulate at least one category of such tests: those that measure multiple genes, proteins or other pieces of clinical information taken from a patient and then use an algorithm or software program to analyze the data.
Highly specific and sensitive assays for serum-based protein biomarkers of lung cancer progression based on selective reaction monitoring (SRM) mass spectrometry are entering the clinic [ 33] and chip-based ELISA platforms have been described that can simultaneously measure multiple serum protein biomarkers from tiny samples of peripheral blood [ 34].
Here, we show that it is possible to detect and measure multiple embryo-derived proteins in single human blastocoels and that some of the proposed targets may provide valuable information regarding the developmental potential of the embryo.
More suggestions(15)
measure multiple axes
measure serum proteins
measure other proteins
measure multiple facets
measure multiple steroids
measure individual proteins
measure antigenic proteins
measure total proteins
measure multiple outcomes
measure surfactant proteins
measure multiple analytes
measure multiple wavelengths
measure multiple parameters
measure multiple symptoms
measure multiple probes
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