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This piece of equipment has never been used to measure mitochondrial function in SS.
It also discusses the use of these methodologies to measure mitochondrial function in physiology and the change in function observed in pathology.
There are many methods to measure mitochondrial function and dysfunction in these systems.
A recently developed high-throughput respirometric assay for mitochondrial biogenesis used the Seahorse Bioscience analyzer to measure mitochondrial function in real time.
Therefore, we asked whether SAL shows the same effect on the mitochondria of cells with stem cell-like features and used HMLE-Twist cells to measure mitochondrial function.
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In this protocol, we sequentially measured mitochondrial function under various metabolic conditions [17].
At 24-h post-exposure, a standard MTS assay (Promega, Madison, Wisconsin) measuring mitochondrial function was performed to determine cell viability.
One caveat to take into consideration when considering these results is that the MTT assay measures mitochondrial function and thus is an indirect measure of proliferation, thus if a primary effect of these treatments is an increase in mitochondrial respiration, then this may exaggerate the conclusion of proliferative effects of this treatment.
More recent work from the same group demonstrated that change in metabolic state alters measured mitochondrial function [ 32].
We measured mitochondrial function under basal and stress conditions in SH-SY5Y cells silenced for PINK1 expression by using an extracellular oxygen flux analyzer.
For measuring mitochondrial function in intact cells and isolated mitochondria, the Seahorse Flux analyzer (Seahorse Biosciences) has revolutionized mitochondrial studies in terms of enabling high-throughput measurement of mitochondrial metabolism (Kembro et al. 2013; Qian and Van Houten 2010).
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