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Owing to the extremely fast kinetics, it was not possible to measure kinetic parameters for most of the APL, but in the few instances where off-rates could be estimated, they correlated with equilibrium dissociation constant measurements.
The values obtained in DSC experiments are used to measure kinetic parameters of the reaction using the equations given in this article.
Potential step chronocoulometric methods were used to measure kinetic parameters for the oxidation of ferrocene at platinum vitreous carbon electrodes in acetonitrile.
Although this assay cannot be used to measure kinetic parameters, a comparison of interaction intensities detected on peptide arrays with published dissociation constants between several effectors and various histone ligands, a general pattern emerges suggesting that interactions that are ≤150 300 uM Kd range can be detected on the arrays (Fig. 3; Table S3; data not shown).
The observation that Ch-MAL shows maximum activity at 70°C, prompted us to measure kinetic parameters at this temperature.
However, the limited sensitivity of these techniques has frustrated attempts to accurately measure kinetic parameters for substrates with low micromolar Km values (23, 41).
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Computer simulations of the global catalytic model using the measured kinetic parameters produced results that mimicked the measured outcomes, and this provided further insights on the catalytic behaviors of the chimeras and basis of their increased NO dioxygenase activities.
Hence, it is fundamental that the rate constants used in the model arise from directly measured kinetic parameters rather than rely on equilibrium data such as affinity measurements.
The recommendations for measuring kinetic parameters are reported together with the benefits/disadvantages using the three mentioned approaches.
From the combinations of the measured kinetic parameters, we inferred that one of the two charge carriers moved much faster than the other.
The model incorporates recently measured kinetic parameters, intra- and extra-particle heat transfer, and particle shrinkage as gaseous products are formed.
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