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The mean fluorescence (channel 1) was used as a measure for cell surface-associated Esp.
As a measure for cell viability, we quantified ATP contents by luciferin-based chemiluminescence.
In general, cell metabolic activity is determined as an indirect measure for cell vitality and proliferation.
We assessed cell viability as a measure for cell number using the CellTiter-Blue assay at different time points.
A SRB assay was used to measure the total amount of protein as a measure for cell proliferation.
The values were background-subtracted and a compound measure for cell viability was calculated as described below.
Similar(47)
The width and contrast of the resonances were measured for cell lengths as low as 100 microm.
DNA amount in cells is often the single parameter measured for cell cycle studies by flow cytometry.
Absorbance at 563 nm was measured for cell viability.
Triplicate wells were measured for cell invasion in each treatment group.
Triplicates declare no conflict of interest.
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