Sentence examples for measure fluorescence at from inspiring English sources

The protein so obtained was immediately used to measure fluorescence at wavelengths of 485 nm for excitation and 530 nm for emission using Varioskan Flash Multimode Reader (Thermo Scientific, USA).

Twenty ul of liposome solution was added to Mtb-infected macrophage cultures, incubated for 16 h, washed twice with PBS and then lysed with 1% SDS PBS for 10 min. Lysates were centrifuged and supernatant were collected to measure fluorescence at excitation 480 nm and emission 530 nm.

Flow cytometry (BD Biosciences, San Jose, CA, USA) was used to measure fluorescence at an excitation wavelength of 502 nm and an emission wavelength of 530.

Local background correction was performed by moving the mask to measure fluorescence at a nearby region, and this value was subtracted from the ciliary Smo fluorescence.

After incubation for 1 h at 37 °C, plates were immediately placed in a Biotek microplate reader to measure fluorescence at 485 excitation and 528 emission.

Estimation of expressed green fluorescent protein (GFP) was quantitatively carried out by measuring fluorescence at NanoDrop ND-3300 spectrofluorometer.

DNA was quantified using Picogreen fluorescent dye (Molecular Probes Inc., Eugene, OR) by measuring fluorescence at 535 nm in a Tecan fluorimeter (Tecan, Crailsheim, Germany).

After amplification a melting curve analysis was performed by increasing the temperature by 0.5°C increments from 55°C to 95°C and measuring fluorescence at each temperature for a period of 10 s.

The bound RNA was quantified by measuring measuring fluorescence at 521 nm.

The bound RNA was quantified by measuring fluorescence at 521 nm.

The cells were analysed with a FACSort flow cytometer, measuring fluorescence at 488 nm.