Exact(2)
There is currently no easy way to directly measure fiber density.
Diabetic persons previously thought not to have neuropathy may have subtle abnormalities with patch skin biopsy, which allows immunohistochemical staining of epidermal nerve fibers to measure fiber density and to examine morphological abnormalities, a measure of small nerve fibers (12, 13).
Similar(58)
Portal tracts (PTs) with immunoreactive fibers were counted in each section and computer-assisted morphometric analysis (Image Pro Plus) was used to measure nerve fiber density (number of immuno-positive nerve fibers/mm (40x)).
The mean value (the percentage of neuronal immunofluorescenc area) based on 8 microscopic fields was determined in 4 rats to obtain a measure of fiber density for comparison of the concave and convex sides of each tibia.
Additionally, another diagnostic technique that has recently become widely and commercially available is the skin punch biopsy, which is used to measure epidermal nerve fiber density (ENFD) [ 6].
The GDNF DS and NGF DS groups were also similar to the nerve isograft group in measures of nerve fiber density, percent neural tissue and myelinated area measurements, but not in terms of total fiber counts.
All other myelinated fiber measures, nonmyelinated fiber densities (Kruskal-Wallis = 1.78, P = 0.64), and Schwann cells density (Kruskal-Wallis = 1.43, P = 0.70) were not different among groups.
Secondary outcomes included a responder analysis, quality of life and pain measures, and intraepidermal nerve fiber density.
The gold standard for assessing small nerve fiber degeneration is the morphometry of intraepidermal nerve fiber density measured by skin punch biopsy, which is an invasive and painful technique (32).
To elucidate whether this effect was associated with an increase in monoaminergic fiber density, we measured the density of TH+, 5-HT+, and NET+ fibers in the PFC of C57BL/6 and β 2−/− mice.
The fiber density as a measure could not be taken into consideration in our study due to the use of the concentric needle because the recorded spikes are often a composite of the activities of more than one muscle fiber.
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