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A custom NimbleGen 12x135K microarray designed from D. serrata expressed sequence tags (ESTs) (Frentiu et al. 2009) was used to measure expression of 11,631 ESTs (supplementary file S3, Supplementary Material online).
Quantitative PCR was performed to verify microarray data and measure expression of additional genes not present on the microarray.
Manatee specific oligonucleotide primers for iNOS were designed to measure expression of relative amounts of mRNA in LPS-stimulated manatee PBMC from captive manatees.
At the end of each culture period, the media were pooled to measure hormone levels, and the cultured follicles were collected to measure expression of cell cycle regulators and steroidogenic enzymes.
In this report the expression of the ubiquitin-proteasome pathway in rectus abdominis muscle has been determined in cancer patients with weight loss of 0 34% using a competitive reverse transcriptase polymerase chain reaction to measure expression of mRNA for proteasome subunits C2 and C5, while protein expression has been determined by western blotting.
High-density DNA microarrays measure expression of large numbers of genes in one assay.
To measure expression of the yhcS and ywpE genes, transcriptional fusions between their promoter regions and the lacZ reporter gene were constructed.
Custom microarrays designed to measure expression of genes thought to play a role in the pathogenesis of asthma have proved useful in establishing the biological relevance of these genes [70].
Therefore, dependent on the region in the gene where the probes are binding to measure expression of a reference gene, profound differences among conditions can be the result of alternatively spliced or terminated transcript variants.
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They profiled gene expression by measuring tens of thousands of RNAs in each sample.
The effect of andrographolide on LPS/IL-4 activated murine macrophages was evaluated by measuring expression of activation relevant cytokines.
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