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To evaluate whether the expressed variants are important for community function, it will be necessary to measure expression levels over time in conjunction with process measurements.
It is not clear why the correlations seen by other labs were not observed in this study, although differences in the methods include the use of only E. coli target proteins, the target proteins only included proteins with C-terminus in the cytoplasm, the proteins were expressed as GFP fusion protein, and GFP fluorescence was used to measure expression levels.
To investigate the developmental expression patterns of catechin synthetic genes, CHS1, CHS2, CHS3, LAR, ANS, ANR1 and ANR2 in C. sinensis, qPCR was performed using gene-specific primers to measure expression levels (Fig. 4).
We next used qRT-PCR to measure expression levels (Fig. 5).
Microarray experiments permit biologists to concurrently measure expression levels of thousands of genes in a single experiment through the hybridization of nucleic acid to pre-designed oligos.
CAGE is a high-throughput method to measure expression levels of a gene and is an essential resource for profiling transcriptional starting sites [15], [16].
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This measures expression levels of 196 human genes known to be differentially expressed in breast cancers or relevant to estrogen receptor signaling.
We conclude that microarrays remain useful and accurate tools for measuring expression levels, and RNA-Seq complements and extends microarray measurements.
We also measured expression levels of classical HLA genes in eight HLA homozygous reference cell lines at baseline and after three treatments (heat shock, LPS, and IFN-gamma).
Measured expression levels are all relative to these references.
The 430A 2.0 Array measures expression levels of 14,000 characterized genes.
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improves expression levels
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