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Due to background, microarray platforms often cannot reliably measure expression in the low-intensity range.
To measure expression in embryos, ca. 20 gravid adult worms were dissected in PBS and the embryos transferred to TRIZOL.
In this study, the microarrays were used to measure expression in 79 different tissues, each in two repetitions.
To measure expression in the germ line, worms were dissected in PBS and RNA was extracted from 6 isolated germ lines for each condition assayed.
In addition to mean data, quantitative measures of distributions across a population can be obtained using techniques such as flow cytometry that measure expression in single cells.
Because tiling arrays measure expression in the intergenic regions of annotated genomes, they can be used to identify and predict operon structures in bacteria.
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Using the re-array, we measured expression in triplicate for 10 different rice organs (Figure 2A and S4).
Because competitive methods require a comparison between results within a gene set to those outside the gene set, these tests cannot be applied in a study that only measured expression in a particular candidate pathway or gene set.
For measuring expression in heart the average correlation is 0.85 with 454-sequencing and 0.98 with cDNA microarray.
Whereas half-sector analysis measured events throughout the duration of an entire cell cycle, the FISH method measured expression in a snapshot of time.
The system is capable of measuring gene expression with accuracy similar to qPCR, yet nCounter® requires significantly less preparatory work and is highly automated and capable of measuring expression in crude cell lysates.
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