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Randomized, controlled exercise trials designed to measure endothelial function, inflammatory markers, sympathetic neural activation, and skeletal muscle metabolism and structure have further defined the pathophysiology, documented the impact of exercise training on these processes, and confirmed the benefit of this therapy.
There are many ways to measure endothelial function.
Finally, FMD makes it possible to repeatedly measure endothelial function in individual rats.
Vascular ultrasound of the brachial artery under conditions of FMD after reactive hyperemia (endothelium-dependent vasodilatation) and in response to glyceryl trinitrate (endothelium-independent vasodilatation) are noninvasive techniques to measure endothelial function.
It is increasingly being used to measure endothelial function as a cardiovascular risk assessment tool in ambulatory patients [ 12- 16], including in the third-generation Framingham Heart Study cohort [ 17].
As the primary end point, we selected to measure endothelial function as flow-mediated dilation (FMD), and included assessments of plasma lipids, BP and pulse wave velocity (PWV) as secondary end points.
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On 4 consecutive days, we measured endothelial function by brachial artery ultrasound in all participants and by pulsewave measurements in a subgroup.
Flow-mediated dilation (FMD) measures endothelial function by inducing a temporary ischemia in a brachial artery and observing the amount of vasodilation following the stressor event.
We performed a maximal exercise test (VO2max) and measured endothelial function using venous occlusion plethysmography and intra-arterial infusion of acetylcholine, sodium nitroprusside and NG-monomethyl-l-arginine.
Among the several non-invasive methods of measuring endothelial function, flow-mediated dilation (FMD), laser Doppler flowmetry, and digital pulse amplitude tonometry (PAT) are the most widely adopted [16 18].
Venous occlusion plethysmography has been found to be a valid method of measuring endothelial function [ 12].
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