We measured two different proteins, which are indicators of cell growth, PCNA, and cyclin D1.
In the next section we fix the parameters to their optimal values found here and compare the HSE and CN measures on different proteins.
The large discrepancy between protein levels of suggested markers, measured with two different proteins assays, raises the question of using absolute values when comparing studies in which different analytic methods have been used.
As expected, the results shown in Figure 2 illustrates that the percentage of essential proteins identified by PeC is consistently higher than that explored by fifteen other centrality measures for the different proteins between them.
MILLIPLEX MAP kits (Millipore Corporation) were used for microsphere-based multiplex immunoassays to measure the concentration of different proteins [25].
This is a measure of how well different proteins are digested by the body, based on the solubility of the amino acids in the protein.
Of the 18 different proteins measured, only phospho-GSK3βwas increased at all time points following nicotine exposure (Fig. 4c, Additional file 1: Data 2 for all phosphorylation data).
One of the advantages of biochemical analyses of poly(GP) burden is the ability to measure different forms of the protein in a quantitative manner.
To measure the expression levels of different proteins using western blot analysis, the animals were killed after 4 h of drug treatment.
An approach called RNA-Seq was then employed to measure the levels of the different mRNA molecules in the cells, and a device called a mass spectrometer was used to identify and measure the levels of many different proteins.
